Eppendorf twin.tec real-time PCR Plates offer all the advantages of regular twin.tec plates and give you the advantage of white wells for your real-time PCR. The limiting factor in low volume real-time PCR is often the remaining intensity of fluorescence. The white wells of the Eppendorf twin.tec real-time PCR Plates reflect fluorescence much better than clear or frosted wells. Thus, lower levels of fluorescence are still detectable with the same instrument – just by using the right consumables. Additionally, white wells significantly reduce interfering background fluorescence and lead to increased homogeneity of replicates and reproducible results. The rigidity of the polycarbonate frame ensure easy and reliable handling – manually or automated. The polypropylene wells guarantee excellent and fast temperature transfer to the sample.
High intensity of fluorescence improves sensitivity in qPCR. This graph shows you an up to 10-fold higher fluorescence measurement of twin.tec real-time PCR plates than those with frosted wells.
Eppendorf twin.tec® real-time PCR plates reduce Ct-values more effectively than other tested plates. On average, Ct-values occur 0.8 cycles earlier (compared to clear wells), indicating better sensitivity.
White wells improve homogeneity of results over the block. In this graph you can see the reduced Standard Deviation over the block compared to other plates.