CCCadvanced FN1 motifs consumables are classical Eppendorf Cell Culture Consumables, additionally coated with a defined synthetic animal- and human-component-free substrate. The substrate (fibronectin-derived motifs with optimized steric configuration) is designed to mimic the cell attachment site of native ECM.
Learn more about cultivation of human iPSCs and MSCs on FN1 motifs and smart features of our cultureware below.
iPSCs: Efficient long-term expansion in a completely synthetic culture system
› Supports efficient long-term hiPSC expansion in a completely defined, animal-, and human-component-free culture system for 25 passages
› Consistent and robust growth rate
› Typical morphology remains stable (Fig. 1)
› hiPSCs remain undifferentiated and maintain functional pluripotency
› Maintenance of trilineage differentiation potential after long-term expansion while exhibiting normal genomic integrity
› For further details see Application Note 389 under “Notes & Papers” below
MSCs: Animal-component-free expansion of human mesenchymal stem cells
› Supports efficient hMSC proliferation in a completely animal-component-free environment even on long term (up to 10 passages)
› Characteristic morphology (Fig. 2) remains stable for 10 successive passages without signs of replicative senescence
› Stable and robust proliferation rate
› Validated with hMSC from different tissue origins
› Undifferentiated hMSCs retain their multi-lineage differentiation potential after expansion
› For further details see Application Note 390 under “Notes & Papers” below
Optimized microscopical performance
The enhancement of the planarity, superior material clarity and reduction of the meniscus of liquids in the wells facilitate every step where manual or automated read-out is necessary. Shadow interference in imaging area is significantly reduced as shown in comparison to a competitor plate.
A pronounced corrugation of the plate lid and a smaller lid makes working with Eppendorf Cell Culture Plates extremely comfortable and safe.